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Journal of Applied Life Sciences International

Journal of Applied Life Sciences International, 2394-1103,Vol.: 14, Issue.: 1


Development of a Universal IPMA Test to Detect Lawsonia Antibodies in Multiple Species Using Protein A and G


A. S. H. Hassenin1*, C. J. Gebhart2 and S. M. Goyal2

1Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, 44519, Egypt.

2College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota 55108, USA.


Article Information
(1) J. Rodolfo Rendón Villalobos, Department of Technological Development, National Polytechnic Institute, México.
(2) Rohit Saluja, Department of Dermatology and Allergy, Charité – Universitätsmedizin Berlin, Germany.
(1) Om Parkash, Shah Abdul Latif University, Pakistan.
(2) Annapurna S. Agasthya, BFIT Group of Institutions, India.
(3) V. Bhanu Rekha, Rajiv Gandhi Institute of Veterinary Education and Research Pondicherry, India.
(4) Sabrina Dominici, University of Urbino, Italy.
(5) Silvia Giono, Instituto Politécnico Nacional, Mexico.
Complete Peer review History: http://www.sciencedomain.org/review-history/21316




A recombinant protein combining the immunoglobulin binding sites of Proteins A and G, conjugated with horseradish peroxidase was used as a universal detection reagent for the assessment of antibodies against Lawsonia intracellularis. The reagent was applied in an indirect immunoperoxidase method in microplates for detection of antibodies to smooth lipopolysaccharide antigen in sera from horse and pig. Also, using protein G and protein A separately which detected from results that is species specific. An added advantage was that a universal conjugate using the proteins A and G detection reagent could be established.


Keywords :

Antibody; protein A/ G; ileitis; imunoperoxidase assay; Lawsonia intracellularis; horses; pig; proliferative enteropathy.


Full Article - PDF    Page 1-7    Article Metrics


DOI : 10.9734/JALSI/2017/32366

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