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Current Journal of Applied Science and Technology, ISSN: 2457-1024; 2231-0843 (old),Vol.: 23, Issue.: 5

Original-research-article

Molecular Identification and Characterization of Alphasatellites Associated with Okra Enation Leaf Curl Virus

 

Gurpreet Kaur1, Navraj Kaur Sarao1, Abhishek Sharma2* and Irfan Khan3

1School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, India.

2Department of Vegetable Science, Punjab Agricultural University, Ludhiana, Punjab, India.

3Department of Plant Pathology, Punjab Agricultural University, Ludhiana, Punjab, India.

Article Information
Editor(s):
(1) Farjana Sultana, College of Agricultural Sciences, International University of Business Agriculture and Technology (IUBAT University), Bangladesh.
(2) Ya-mei Gao, College of life science and technology, Heilongjiang Bayi Agariculture University, Heilongjiang, China.
Reviewers:
(1) Rajarshi Kumar Gaur, Mody University of Science and Technology, India.
(2) Davi Coe Torres, Instituto Nacional de Câncer (INCA), Brazil.
(3) Addolorata Colariccio, Instituto Biológico São Paulo, Brazil.
(4) Khadim Hussain, Government College University Faisalabad, Pakistan.
Complete Peer review History: http://www.sciencedomain.org/review-history/21139

Abstracts

Symptomatic okra plants showing leaf curling, typical yellowing and thickening of veins were collected and characterized. Full genome of the virus was amplified using phi-29 polymerase exploiting rolling circle amplification (RCA) mechanism. The concatemer was digested with restriction enzyme and cloned in a vector for sequencing. Sequence analysis and nucleotide (ntd) comparison revealed that the viral genome is of 2,741 bp in length and showed the highest nucleotide identities of 91-99% with Okra enation leaf curl virus (OELCuV) which has spread in all okra growing region in India. The plants possessing OELCuV has also shown association of alphasatellites. Four out of six alphasatellites characterized showed nucleotide similarity with Sida yellow vein China alphasatellite which has been earlier reported from Nepal and China. Two alphasatellite molecule showed highest identity with Gossypium darwinii symptomless alphasatellite (GDarSLA) and Gossypium mustelinium symptomless alphasatellite (GMusSLA) each, respectively.

Keywords :

Alphasatellites; begomovirus; disease; phylogenetic analysis.

Full Article - PDF    Page 1-9

DOI : 10.9734/CJAST/2017/35109

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