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Annual Research & Review in Biology

Annual Research & Review in Biology, ISSN: 2347-565X,Vol.: 17, Issue.: 1

 

Original-research-article

 

Variability and Genetic Structure of a Natural Population of Trichoderma spp. Isolated from Different Substrates in Morocco

 

 

Wafa Khirallah1, Amina Ouazzani Touhami1, Ghizlane Diria2, Fatima Gaboun2, Rachid Benkirane1 and Allal Douira1*

1Laboratoire de Botanique, Biotechnologie et Protection des Plantes, Département de Biologie, Faculté des Sciences, Université Ibn Tofail, BP. 133, Kénitra, Morocco.

2Biotechnology Unit, Institut National de Recherche Agronomique (INRA), Av Victoire BP 415, Rabat 10000, Morocco.

 

Abstracts

 

Ten species of Trichoderma antagonists have been collected from different ecosystems (forest soils, compost and strawberry plants) and characterized according to their molecular tools. Molecular characterization was performed by amplifying and analyzing the sequences of the internal transcribed separator coding 1 and 4 (ITS). Trichoderma isolates were identified as Trichoderma asperellum, more than 90% similarity, except the SDLA2 strain gave Trichoderma harzianum species (The isolate SDLA2 was closely related at the nucleotide level to the China’s isolate gi|748053410|gb|KM277992.1| Trichoderma harzianum, 100% of similarity). The dissimilarity matrix based on ITS analysis showed that the Trichoderma isolates: SELM4, SMAA6, SMAA8 and SDLA27 were the less dissimilar (1.3% to 1.6%), while the isolate SDLA2 with the others isolates, except with the isolate gi|748053410|gb|KM277992.1 (1.1%) are the most dissimilar (9.9% to 16.3%).

 

Keywords :

Trichoderma asperellum; T. harzianum; molecular character; similarity; dissimilarity; DNA extraction; ITS.

 

Full Article - PDF    Page 1-11    Article Metrics

 

DOI : 10.9734/ARRB/2017/35389

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