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Journal of Advances in Medicine and Medical Research, ISSN: 2456-8899, ISSN: 2231-0614 (Past),Vol.: 23, Issue.: 10

Original-research-article

Lymphocyte Transformation and Nitro-blue Tetrazolium Reduction Rate of Neutrophils among HIV Infected Adults in Sokoto Metropolis

 

Abdulmumini Yakubu1 and Mustapha Umar Kalgo2*

1Department of Internal Medicine, Usmanu Danfodiyo University/Teaching Hospital, Sokoto, Nigeria.

2Department of Immunology, School of Medical Laboratory Sciences, Usmanu Danfodiyo University, P.M.B 2346 Sokoto, Nigeria.

Article Information

Editor(s):

(1) Thomas I. Nathaniel, University of South Carolina, School of Medicine-Greenville, Greenville, USA.

Reviewers:

(1) Karpal Singh Sohal, Muhimbili University of Health and Allied Sciences, Tanzania.

(2) Neema Tiwari, Era’s Luck Now Medical College and Hospital, India.

Complete Peer review History: http://www.sciencedomain.org/review-history/20902

Abstracts

Aim: The study was aimed at evaluating the functional activity of lymphocytes and neutrophils among HIV positive adults in Sokoto, Nigeria.

Study Design: This was a cross sectional comparative study of HIV positive subjects on HAART, HAART Naïve and apparently healthy control participants.

Methodology: A total of 157 adults were recruited for the study comprised of90 HIV seropositive subjects and 67 age and sex-matched apparently healthy controls.The subjects (HIV infected participants) were further sub-grouped into four different groups based on the revised WHO standard criteria for staging of HIV/AIDS infected adults; as Clinical stage I (n=31), Clinical stage II (n=25), Clinical stage III (n=19) and Clinical stage IV (n=15).The CD4+ cells were evaluated using flow cytometric method, percentage transformed cells were evaluated using culture techniques and neutrophil phagocytic activity was determined using Nitro- Blue Tetrazolium reduction test (NBT). Data were analyzed using SPSS Version 20. A p-value ≤ 0.05 was considered statistically significant.

Result: The CD4+ count, percentage lymphocyte transformation and Neutrophil ingestion rate of NBT were significantly lowered in HIV infected subjects compared with controls (p<0.05). The CD4+ count, percentage transformed cells and formazan generated by neutrophil was significantly higher among female subjects compared with male subjects (p<0.05).

Conclusion: Our findings revealed a lowered blast formation and neutrophil ingestion rate of NBT indicating a functional derangement in the innate and adaptive immune responses.

Keywords :

Lymphocyte transformation; neutrophils; NBT; CD4+; HIV; Sokoto.

Full Article - PDF    Page 1-9

DOI : 10.9734/JAMMR/2017/36033

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