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Journal of Advances in Medicine and Medical Research, ISSN: 2456-8899, ISSN: 2231-0614 (Past),Vol.: 23, Issue.: 10


Lymphocyte Transformation and Nitro-blue Tetrazolium Reduction Rate of Neutrophils among HIV Infected Adults in Sokoto Metropolis


Abdulmumini Yakubu1 and Mustapha Umar Kalgo2*

1Department of Internal Medicine, Usmanu Danfodiyo University/Teaching Hospital, Sokoto, Nigeria.

2Department of Immunology, School of Medical Laboratory Sciences, Usmanu Danfodiyo University, P.M.B 2346 Sokoto, Nigeria.

Article Information


(1) Thomas I. Nathaniel, University of South Carolina, School of Medicine-Greenville, Greenville, USA.


(1) Karpal Singh Sohal, Muhimbili University of Health and Allied Sciences, Tanzania.

(2) Neema Tiwari, Era’s Luck Now Medical College and Hospital, India.

Complete Peer review History:


Aim: The study was aimed at evaluating the functional activity of lymphocytes and neutrophils among HIV positive adults in Sokoto, Nigeria.

Study Design: This was a cross sectional comparative study of HIV positive subjects on HAART, HAART Naïve and apparently healthy control participants.

Methodology: A total of 157 adults were recruited for the study comprised of90 HIV seropositive subjects and 67 age and sex-matched apparently healthy controls.The subjects (HIV infected participants) were further sub-grouped into four different groups based on the revised WHO standard criteria for staging of HIV/AIDS infected adults; as Clinical stage I (n=31), Clinical stage II (n=25), Clinical stage III (n=19) and Clinical stage IV (n=15).The CD4+ cells were evaluated using flow cytometric method, percentage transformed cells were evaluated using culture techniques and neutrophil phagocytic activity was determined using Nitro- Blue Tetrazolium reduction test (NBT). Data were analyzed using SPSS Version 20. A p-value ≤ 0.05 was considered statistically significant.

Result: The CD4+ count, percentage lymphocyte transformation and Neutrophil ingestion rate of NBT were significantly lowered in HIV infected subjects compared with controls (p<0.05). The CD4+ count, percentage transformed cells and formazan generated by neutrophil was significantly higher among female subjects compared with male subjects (p<0.05).

Conclusion: Our findings revealed a lowered blast formation and neutrophil ingestion rate of NBT indicating a functional derangement in the innate and adaptive immune responses.

Keywords :

Lymphocyte transformation; neutrophils; NBT; CD4+; HIV; Sokoto.

Full Article - PDF    Page 1-9

DOI : 10.9734/JAMMR/2017/36033

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