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Asian Journal of Medicine and Health

Asian Journal of Medicine and Health, 2456-8414,Vol.: 6, Issue.: 3

Original-research-article

Anti-oxidative and Anti-inflammatory Activities of Three Triterpenoid Acids in Human Bronchial Epithelial BEAS-2B Cells

 

Shih-Ming Tsao1,2†, Mei-Chin Yin3*† and Zhi-Hong Wang3*

1Institute of Medicine, Chung Shan Medical University, Taichung City, 408, Taiwan.

2Sections of Infectious Diseases and Chest Medicine, Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung City, 408, Taiwan.

3Department of Nutrition, China Medical University, Taichung City, 404, Taiwan.

 

Article Information

Editor(s):

(1) Satesh Bidaisee, Health and Preventive Medicine, St. George's University, University Centre, West Indies.

Reviewers:

(1) Piotr Michel, Medical University of Lodz, Poland.

(2) Wei Hsum Yap, Taylor’s University, Malaysia.

(3) Ming-An Ouyang, Fujian Agriculture and Forestry University, China.

Complete Peer review History: http://www.sciencedomain.org/review-history/20449

 

Abstracts

 

Protective effects of three triterpenoid acids, asiatic acid (AA), glycyrrhizic acid (GA) or oleanolic acid (OA), for BEAS-2B cells, human bronchial epithelial cells, against hydrogen peroxide (H2O2) induced injury were examined. Cells were pre-treated with AA, GA or OA at 4 or 8 μmol/l for 48 hr at 37°C, and followed by 100 μM H2O2 treatment for 4 hr at 37°C. Cell viability, mitochondrial membrane potential, oxidative, apoptotic and inflammatory factors were measured. Western blot analysis was used to determine protein expression. Results showed that H2O2 treatment reduced both Na+-K+-ATPase activity and mitochondrial membrane potential of BEAS-2B cells. Pre-treatments from triterpenoid acids maintained Na+-K+-ATPase activity and mitochondrial membrane potential. H2O2 stimulated the formation of reactive oxygen species, interleukin-6, tumor necrosis factor-α and prostaglandin E2 in BEAS-2B cells. Three triterpenoid acids pre-treatments lowered the levels of these factors. H2O2 enhanced the protein expression of p47phox, gp91phox, nuclear factor-kB (NF-kB) and p-p38. AA, GA or OA pre-treatments suppressed the expression of p47phox, NF-kB p65 and p-p38; but only at 8 μmol/l decreased gp91phox expression. These findings suggest that these triterpenoid acids are potent protective agents for bronchial epithelial cells to mitigate oxidative and inflammatory injury.

 

Keywords :

Triterpenoid acids; bronchial epithelial cells; ROS; NF-kB; Na+-K+-ATPase activity.

 

Full Article - PDF    Page 1-11    Article Metrics

 

DOI : 10.9734/AJMAH/2017/35271

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