Quick Menu

Upcoming Journals
  • No Upcoming Journals

Asian Journal of Medicine and Health

Asian Journal of Medicine and Health, 2456-8414,Vol.: 6, Issue.: 3

 

Original-research-article

 

Anti-oxidative and Anti-inflammatory Activities of Three Triterpenoid Acids in Human Bronchial Epithelial BEAS-2B Cells

 

 

Shih-Ming Tsao1,2†, Mei-Chin Yin3*† and Zhi-Hong Wang3*

1Institute of Medicine, Chung Shan Medical University, Taichung City, 408, Taiwan.

2Sections of Infectious Diseases and Chest Medicine, Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung City, 408, Taiwan.

3Department of Nutrition, China Medical University, Taichung City, 404, Taiwan.

 

Abstracts

 

Protective effects of three triterpenoid acids, asiatic acid (AA), glycyrrhizic acid (GA) or oleanolic acid (OA), for BEAS-2B cells, human bronchial epithelial cells, against hydrogen peroxide (H2O2) induced injury were examined. Cells were pre-treated with AA, GA or OA at 4 or 8 μmol/l for 48 hr at 37°C, and followed by 100 μM H2O2 treatment for 4 hr at 37°C. Cell viability, mitochondrial membrane potential, oxidative, apoptotic and inflammatory factors were measured. Western blot analysis was used to determine protein expression. Results showed that H2O2 treatment reduced both Na+-K+-ATPase activity and mitochondrial membrane potential of BEAS-2B cells. Pre-treatments from triterpenoid acids maintained Na+-K+-ATPase activity and mitochondrial membrane potential. H2O2 stimulated the formation of reactive oxygen species, interleukin-6, tumor necrosis factor-α and prostaglandin E2 in BEAS-2B cells. Three triterpenoid acids pre-treatments lowered the levels of these factors. H2O2 enhanced the protein expression of p47phox, gp91phox, nuclear factor-kB (NF-kB) and p-p38. AA, GA or OA pre-treatments suppressed the expression of p47phox, NF-kB p65 and p-p38; but only at 8 μmol/l decreased gp91phox expression. These findings suggest that these triterpenoid acids are potent protective agents for bronchial epithelial cells to mitigate oxidative and inflammatory injury.

 

Keywords :

Triterpenoid acids; bronchial epithelial cells; ROS; NF-kB; Na+-K+-ATPase activity.

 

Full Article - PDF    Page 1-11    Article Metrics

 

DOI : 10.9734/AJMAH/2017/35271

Review History    Comments

Search this site

Advanced Search

Announcement & News

ISI Thomson Reuters selected British Journal of Pharmaceutical Research for Emerging Sources Citation Index

We are delighted to inform that ISI Thomson Reuters selected British Journal of Pharmaceutical Resea...

SCOPUS selected Annual Research & Review in Biology (ARRB)

We are delighted to inform that famous indexing organization SCOPUS (from Elsevier) selected  A...

Index Copernicus Evaluation Result Released

We are delighted to inform that Index Copernicus (a leading indexing organization from Pol...

Journal Repository (JR): Permanent Digital Archiving of SDI journals

SDI is happy to announce that all our journals are now permanently archived in Journal Repository (J...

Index Copernicus Value of British Journal of Medicine and Medical Research

  We are delighted to inform that Index Copernicus (a leading indexing organization ...

SDI journal got 35th ranking in Publons

We are delighted to announce (as of 04/01/2016) that British Journal of Medicine and Medical Re...

Growth of SDI and world publication market

As of 2014, total 25,064 journals are competing in World market of journal publication. In 2011, tot...

Publication and site statistics (up to Sept, 2015)

  Number of submission: 22181 (Total up to Sept, 2015) Number of published paper: 10949&n...

Index Medicus selected 15 SDI journals

We are delighted to inform that Index Medicus (IMSEAR) has selected following 15 SDI journals for in...

SCIENCEDOMAIN international received 5844 submissions in 2013

SCIENCEDOMAIN international is pleased to announce that it has received 5844 submissions in 2013, ac...



SCIENCEDOMAIN Awards

  • No Awards listed.

Browser Compatibility : Mozila firefox, Google Crome and IE 7 & above. Creative Commons License Terms & Condition   |   Privacy Policy   |   Join Us   |   Help   |   Contact Us
© Copyright 2010-2017, SCIENCEDOMAIN international. All rights reserved.