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British Journal of Medicine and Medical Research, ISSN: 2231-0614,Vol.: 22, Issue.: 2

Original-research-article

Phenolphthalein Method as a Simple and Low Cost Test for Detection of Fecal Occult Blood

 

Seyedeh Maryam Sharafi1, Azar Balouti2, Mahboobeh Hadipour2, Hajar Yazdani2, Morteza Yousefi3 and Hossein Yousofi Darni4*

1Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

2Department of Medical Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran.

3Department of Surgery, Kurdistan University of Medical Sciences, Sanandaj, Iran.

4Cancer Prevention Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran.

Article Information
Editor(s):
(1) Mohammed Rachidi, Molecular Genetics of Human Diseases, French Polynesia, University Paris 7 Denis Diderot, Paris, France.
(2) Gentian Vyshka, University of Medicine in Tirana, Albania.
Reviewers:
(1) Cletus Anes Ukwubile, Federal Polytechnic, Nigeria.
(2) Somchai Amornyotin, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand.
Complete Peer review History: http://www.sciencedomain.org/review-history/20209

Abstracts

Background: Colorectal cancer is one of the main causes of cancer related death worldwide. Different investigation showed that screening with fecal occult blood is able to reduce incidence of colorectal cancer in a given area. To provide a simple and cost effective test for screening of colorectal cancer in this work sensitivity of phenolphthalein method in detection of occult blood has been investigated.

Methods: In this descriptive study alcohol pyramidon and phenolphthalein methods were used to estimate the minimum amount of human occult blood which can be detected in distilled water and fecal samples. Then 100 fecal samples were examined by the two tests.

Results: Alcohol pyramidon method detected minimum amount of 0.125 µL blood in 1 ml distilled water while phenolphthalein methods detected minimum amount of 0.0312 µL blood in 1 mL distilled water. In another experiment Alcohol pyramidon method detected minimum amount of 1 µL blood in 1 mL fecal sample while phenolphthalein methods detected minimum amount of 0.25 µL blood in about 1 mL fecal sample. Phenolphthalein method provided more positive results when 100 fecal samples were tested.

Conclusion: phenolphthalein method is a sensitive, simple and economy test for colorectal cancer screening which can be used both in labs and at home.

Keywords :

Colorectal cancer; occult blood; screening; phenolphthalein method.

Full Article - PDF    Page 1-6

DOI : 10.9734/BJMMR/2017/34677

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