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Annual Research & Review in Biology, ISSN: 2347-565X,Vol.: 14, Issue.: 4


Molecular Detection of New Delhi Metallo Beta Lactamase 1 (NDM-1) Producing Bacterial Isolates in Kano- Northwestern Nigeria


S. A. Abdullahi1*, A. H. Arzai2, I. Yusuf2, S. M. Adamu3, S. Adamu4, Y. A. Koki5, M. A. Rabi’u6 and A. M. Abbas7

1Public Health and Diagnostic Institute, Northwest University Kano, Nigeria.

2Department of Microbiology, Faculty of Sciences, Bayero University, Kano, Nigeria.

3Department of Pathology, National Orthopaedic Hospital Dala, Kano, Nigeria.

4Department of Pharmacy, Infectious Diseases Hospital, Kano, Nigeria.

5Department of Pathology, Murtala Muhammad Specialist Hospital, Kano, Nigeria.

6Jigawa Research Institute, Kazaure, Jigawa, Nigeria.

7Sir Muhammad Sunusi Specialist Hospital, Kano, Nigeria.

Article Information
(1) J. David Puett, Department of Biochemistry and Molecular Biology, University of Georgia, Georgia.
(2) George Perry, Dean and Professor of Biology, University of Texas at San Antonio, USA.
(1) Arun S. Kharat, Dr. Babasaheb Ambedkar Marathwada University, India.
(2) Babatunde Olanrewaju Motayo, Federal Medical Centre, Nigeria.
(3) Payala Vijayalakshmi, Gitam University, India.
(4) Adesoji, A. Timilehin, Federal University Dutsin-Ma, Nigeria.
Complete Peer review History: http://www.sciencedomain.org/review-history/19961


New Delhi Metallo Beta Lactamase 1 (NDM-1) is an enzyme with zinc ions at its active site that cleaves the amide bond of β-lactam ring and provides resistance against major classes of β-lactam antibiotics. The molecular detection of NDM-1 producing bacterial isolates from tertiary Hospitals in Kano was investigated. A total of 500 bacterial isolates of Enterobacteriaceae and Pseudomonas aeruginosa from samples of blood, urine, catheter tip were screened for NDM-1 over a period of 12 months. The isolates were screened preliminarily for carbapenemases using meropenem (10 µg) and imipenem (10 µg) by disc diffusion technique. Isolates of 23 mm and 21 mm for meropenem and imipenem respectively were confirmed by modified Hodge test then EDTA Disc Synergy Test using two meropenem discs, one with MEM (10 µg), and other containing 10 µl of 0.1 M anhydrous EDTA (292 µg) for Metallo Beta Lactamase (MBLs) and finally seventeen MBLs isolates were screened with NDM-1 specific primers by PCR then four PCR positive products were sequenced for blaNDM-1 gene. Of the 500 clinical bacterial isolates screened, 162(32.4%), 43(8.6%) and 4(0.8%) were found to produce carbapenemase, MBLs and NDM-1 respectively. The highest frequency of NDM-1 producers was found among Escherichia coli 3(1.6%) followed by Klebsiella pneumoniae 1(0.5%). Based on clinical samples, blood (25.0%) was found to have highest prevalence of MBLs followed by catheter tips (21.0%), wound swabs (11.1%) and urine (6.3%). Conclusively, NDM-1 was first detected in Kano, Nigeria.

Keywords :

Carbapenemase; Enterobacteriaceae; imipenem; meropenem; New Delhi Metall B-Lactamase-1; PCR.

Full Article - PDF    Page 1-6

DOI : 10.9734/ARRB/2017/33477

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