International Journal of TROPICAL DISEASE & Health, ISSN: 2278-1005,Vol.: 24, Issue.: 2
Prevalence and Molecular Detection of Quinolone-Resistant E. coli in Rectal Swab of Apparently Healthy Cattle in Bangladesh
Md. Montasir Mamun1, Jayedul Hassan1, K. H. M. Nazmul Hussain Nazir1, Md. Alimul Islam1, Khalada Zesmin2, Md. Bahanur Rahman1 and Md. Tanvir Rahman1* 1Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh. 2Veterinary Training Institute, Mymensingh, Bangladesh.
Md. Montasir Mamun1, Jayedul Hassan1, K. H. M. Nazmul Hussain Nazir1, Md. Alimul Islam1, Khalada Zesmin2, Md. Bahanur Rahman1 and Md. Tanvir Rahman1*
1Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
2Veterinary Training Institute, Mymensingh, Bangladesh.
(1) Triveni Krishnan, Division of Virology, National Institute of Cholera and Enteric Diseases, Kolkata, India.
(1) Auwal Sani, Ahmadu Bello University, Nigeria.
(2) Leonardo Basco, Aix-Marseille University, France.
(3) Masaaki Minami, Nagoya City University, Japan.
Complete Peer review History: http://www.sciencedomain.org/review-history/19744
Emergence of antibiotic resistance is a serious health problem both in human and animal all over the world. In this study, we investigated the prevalence of quinolone-resistant E. coli isolated from apparently healthy cattle in Mymensingh district, Bangladesh. A total of 137 rectal swabs was screened among which 95 was found positive for E. coli. Confirmation of isolation of E. coli was done by PCR targeting 16S rRNA gene of E. coli (prevalence 69.3%). Resistance against quinolone is primarily due to activities of qnrS and qnrA gene products. Among these E. coli quinolone-resistant gene qnrS was detected in 11 isolates. None of the isolates were found positive for qnr Agene. The overall prevalence of qnrS positive E. coli was 8.0%. Many of these quinolone-resistant E. coli was multidrug-resistant. Nucleotide sequence analysis of qnrS gene showed homology with the qnrS gene detected in China, Nigeria, Taiwan, Russia, Turkey and USA. All isolates that were resistant to multiple quinolones were found highly sensitive to imipenem, ertapenem and meropenem. The results of this study indicated that apparently healthy cattle harbor quinolone-resistant E. coli which have both clinical and public health significance. If strict regulation on the use of quinolones in food animals is not maintained, these quinolone-resistant E. coli may be transmitted to humans and other animals and may cause serious health problems in future. To the best of our knowledge, this is the first report on the molecular detection of quinolone-resistant E. coli in cattle in Bangladesh.
Escherichia coli; healthy cattle; rectal swab; quinolone resistance; qnrS gene.
DOI : 10.9734/IJTDH/2017/34404Review History Comments