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British Biotechnology Journal, ISSN: 2231-2927,Vol.: 3, Issue.: 4 (October-December)

Original-research-article

Genetic Characterization of Insulin Growth Factor-1 and Its Receptor Genes in Egyptian Buffalo (Bubalus bubalis L.)

 

Othman E. Othman1*, Mohamed F. Abdel-Samad1, Nadia A. Abo El Maaty1 and Karima M. Sewify2

1Cell Biology Department, National Research Center, Dokki, Egypt.
2Zoology Department, Girl Faculty, Ain Shams University, Egypt.

Article Information

Editor(s):

(1) Csilla Tothova, Clinic for Ruminants, University of Veterinary Medicine and Pharmacy in Kosice Slovakia.

Reviewers:

(1) Anonymous.

(2) Anonymous.

(3) Brijesh Sharma, Mangalayatan University, India.

Complete Peer review History: http://www.sciencedomain.org/review-history/1970

Abstracts

Aim: The somatotropic axis (SA) comprises genes associated with economically important quantitative traits in livestock like mammary and muscle growth as well as carcass traits. Insulin growth factor-1 (IGF-1) and its receptor (IGF-1R) are two important genes belonging to the SA. The aim of this study was to evaluate the genetic polymorphism of IGF1/SnaBI and IGF-1R/TaqI restriction sites in Egyptian buffalo.
Methodology: Genomic DNA was extracted from blood samples of 100 healthy buffaloes maintained at the Mahlet Mussa and El-Gmeasa herds from 2010 to 2012. PCR was performed using primers flanking a 250-bp fragment of the regulatory region of the buffalo IGF-1 gene and a 616-bp fragment of the IGF-1R gene encompassing 51-bp from exon 12, 479-bp from intron 12 and 86-bp from exon 13. The PCR-amplified fragments were digested with SnaBI (IGF-1) and TaqI (IGF-1R), electrophoresed and analyzed on agarose gels stained with ethidium bromide. The two amplified fragments were also sequenced and aligned with published sequences.
Results: All buffaloes investigated in this study were genotyped BB (i.e., negative for the SnaBI restriction site at position 224^225 (TAC^GTA) of the IGF-1 regulatory region) and AA (i.e., positive for the TaqI restriction site at position 47^48 (T^CGA) of the IGF-1R exon 12).
Conclusion: Our Egyptian buffaloes were homozygous BB and AA for IGF1/SnaBI and IGF-1R/TaqI restriction sites, respectively. The nucleotide sequence data were submitted to NCBI/Bankit/GenBank with the accession numbers KC852883 and KC852884 for Egyptian buffalo IGF-1 and IGF-1R genes, respectively. The study of variation in these sequences may be useful in future marker-assisted selection (MAS) and genetic conservation programs.

Keywords :

Buffalo; IGF-1; IGF-1R, PCR; RFLP.

Full Article - PDF    Page 592-604

DOI : 10.9734/BBJ/2013/4869

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