British Biotechnology Journal, ISSN: 2231-2927,Vol.: 3, Issue.: 4 (October-December)
Genetic Characterization of Insulin Growth Factor-1 and Its Receptor Genes in Egyptian Buffalo (Bubalus bubalis L.)
Othman E. Othman1*, Mohamed F. Abdel-Samad1, Nadia A. Abo El Maaty1 and Karima M. Sewify2 1Cell Biology Department, National Research Center, Dokki, Egypt.
2Zoology Department, Girl Faculty, Ain Shams University, Egypt.
Othman E. Othman1*, Mohamed F. Abdel-Samad1, Nadia A. Abo El Maaty1 and Karima M. Sewify2
1Cell Biology Department, National Research Center, Dokki, Egypt.
(1) Csilla Tothova, Clinic for Ruminants, University of Veterinary Medicine and Pharmacy in Kosice Slovakia.
(3) Brijesh Sharma, Mangalayatan University, India.
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Aim: The somatotropic axis (SA) comprises genes associated with economically important quantitative traits in livestock like mammary and muscle growth as well as carcass traits. Insulin growth factor-1 (IGF-1) and its receptor (IGF-1R) are two important genes belonging to the SA. The aim of this study was to evaluate the genetic polymorphism of IGF1/SnaBI and IGF-1R/TaqI restriction sites in Egyptian buffalo.
Methodology: Genomic DNA was extracted from blood samples of 100 healthy buffaloes maintained at the Mahlet Mussa and El-Gmeasa herds from 2010 to 2012. PCR was performed using primers flanking a 250-bp fragment of the regulatory region of the buffalo IGF-1 gene and a 616-bp fragment of the IGF-1R gene encompassing 51-bp from exon 12, 479-bp from intron 12 and 86-bp from exon 13. The PCR-amplified fragments were digested with SnaBI (IGF-1) and TaqI (IGF-1R), electrophoresed and analyzed on agarose gels stained with ethidium bromide. The two amplified fragments were also sequenced and aligned with published sequences.
Results: All buffaloes investigated in this study were genotyped BB (i.e., negative for the SnaBI restriction site at position 224^225 (TAC^GTA) of the IGF-1 regulatory region) and AA (i.e., positive for the TaqI restriction site at position 47^48 (T^CGA) of the IGF-1R exon 12).
Conclusion: Our Egyptian buffaloes were homozygous BB and AA for IGF1/SnaBI and IGF-1R/TaqI restriction sites, respectively. The nucleotide sequence data were submitted to NCBI/Bankit/GenBank with the accession numbers KC852883 and KC852884 for Egyptian buffalo IGF-1 and IGF-1R genes, respectively. The study of variation in these sequences may be useful in future marker-assisted selection (MAS) and genetic conservation programs.
Buffalo; IGF-1; IGF-1R, PCR; RFLP.
Full Article - PDF Page 592-604
DOI : 10.9734/BBJ/2013/4869Review History Comments