Quick Menu

Upcoming Journals

International Blood Research & Reviews

International Blood Research & Reviews, ISSN: 2321-7219,Vol.: 7, Issue.: 2

Short Research Article

Molecular Detection of Hepatitis C Virus (HCV) by Conventional One-step RT-PCR Coupled with Nested PCR


Vikrant Sharma1, Deepak Kumar2, Divya Dhull1, Sulochana Kaushik3, Jaya Parkash Yadav3 and Samander Kaushik1*

1Centre for Biotechnology, Maharshi Dayanand University, Rohtak, India.

2Department of Biotechnology and Molecular Medicine, Pt BDS, PGIMS, Rohtak, India.

3Department of Genetics, Maharshi Dayanand University, Rohtak, India.


Article Information
(1) Ricardo Forastiero, Department of Hematology, Favaloro University, Argentina.
(1) Y. J. Peter, University of Abuja, Nigeria.
(2) Lívia Garcia Bertolacci-Rocha, Universidade Federal de Goiás, Brasil.
(3) Shamala Moodley, Mangosuthu University of Technology, South Africa.
Complete Peer review History: http://www.sciencedomain.org/review-history/19547




Aims: HCV causes both acute and chronic infections and can be easily transmitted through contaminated blood or other body fluids. The present study deals with the molecular detection of HCV with help of one-step RT-PCR assay followed by nested PCR and agarose gel electrophoresis. 

Study Design: RNA extracted from the confirmed positive samples of HCV was utilized for the standardization of the one-step RT-PCR assay and nested PCR assay for diagnosis of HCV.

Place and Duration of Study: Centre for Biotechnology, Maharshi Dayanand University, Rohtak Haryana, India, during period of one year (January-December 2015).

Methodology: HCV positive samples were obtained from Department of Medicine, Maulana Azad Medical College (MAMC), New Delhi, India. Published primers from most conserved regions of HCV were taken and these primers were able to amplify all the strains of HCV. One-step RT-PCR kits, primers, extracted RNA from these positive samples were used for standardization of molecular diagnostic assays. The results were checked by 2% agarose gel electrophoresis.

Results: Positive samples of HCV were detected by nested PCR. Positive samples showed sharp band of 405bp while there was no amplification in the negative control.

Conclusion: Rapid tests have low sensitivity and specificity while molecular assays are rapid, sensitive and specific. Conventional one-step RT-PCR assay followed by nested PCR is rapid, specific, sensitive and it is also less costly than real-time RT-PCR. Cost of an assay is an important factor in controlling a disease in resource limited settings of developing countries.


Keywords :

Hepatocellular carcinoma; conventional PCR; viral hepatitis; hepatitis C virus, one-step RT-PCR, nested PCR, molecular diagnosis.


Full Article - PDF    Page 1-6    Article Metrics


DOI : 10.9734/IBRR/2017/34463

Review History    Comments

Search this site

Advanced Search

Announcement & News

ISI Thomson Reuters selected British Journal of Pharmaceutical Research for Emerging Sources Citation Index

We are delighted to inform that ISI Thomson Reuters selected British Journal of Pharmaceutical Resea...

SCOPUS selected Annual Research & Review in Biology (ARRB)

We are delighted to inform that famous indexing organization SCOPUS (from Elsevier) selected  A...

Index Copernicus Evaluation Result Released

We are delighted to inform that Index Copernicus (a leading indexing organization from Pol...

Journal Repository (JR): Permanent Digital Archiving of SDI journals

SDI is happy to announce that all our journals are now permanently archived in Journal Repository (J...

SDI journal got 35th ranking in Publons

We are delighted to announce (as of 04/01/2016) that British Journal of Medicine and Medical Re...

Growth of SDI and world publication market

As of 2014, total 25,064 journals are competing in World market of journal publication. In 2011, tot...

Science (IF: 31) report confirmed the high standard of SDI journal

As per a recent report (Link) of Science journal (present Impact factor 31), one of our journal (Bri...

SDI introduced Post-publication peer review by its comment section

SDI journals encourage Post-publication peer review by its comment section   Policy details a...

SDI promotes transparent Advanced OPEN peer review

We have migrated to transparent and toughest ‘Advanced OPEN peer review’ system (...


  • No Awards listed.

Browser Compatibility : Mozila firefox, Google Crome and IE 7 & above. Creative Commons License Terms & Condition   |   Privacy Policy   |   Join Us   |   Help   |   Contact Us
© Copyright 2010-2018, SCIENCEDOMAIN international. All rights reserved.