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Biotechnology Journal International

Biotechnology Journal International, ....,Vol.: 18, Issue.: 1

 

Original-research-article

 

Antibacterial Potential of Magnesium Oxide Nanoparticles Synthesized by Aspergillus niger

 

 

Emad J. Ibrahem1, Karkaz M. Thalij2* and Amin S. Badawy2

1Sulaimani Directorate of Health, Ministry of Health, Kurdistan, Iraq.

2Department of Food Science, College of Agriculture, Tikrit University, Iraq.

 

Abstracts

 

A total of 280 urinary tract infection samples were collected in this investigation. Out of them 212(75.7%) samples showed a positive response.to bacterial isolates. Morphological, cultural and biochemical testes were confirmed using VITEK 2 System. Its revealed that 54 (30.2%) of the bacterial isolates were gram positive and 158 (69.8%) gram negative. The bacterial isolates were distributed as Escherichia coli 96 (45.2%), followed by Klebsiella pneumonia 48 (22.6%), Staphylococcus aureus 43(20.3%), Pseudomonas aeruginosa 14 (6.6%), and Staphylococcus epidermidis 11 (5.2%). The synthesis of magnesium oxide nanoparticles (MgO NPs) was performed using Aspergillus niger Method. Agar Wells Diffusion Method was applied for the evaluation of antibacterial activity against gram positive S. aureus, and gram negative P. aeruginosa bacteria isolated from Urine tracts infection (UTI). The results showed that the biosynthesized MgO NPs appeared to be an extracellular with a size range of 43-91 nm as confirmed by Scanning Electron Microscopy (SEM) and UV-Visible spectroscopy for the absorbance band at 256.5 nm. Besides, MgO NPs were found to be an effective antibacterial agent against Gram positive S. aureus, and the zone inhibition diameter at 27 mm. Howover, the zone inhibition diameter against gram negative P. aeruginosa bacteria was at 24 mm, compared with inhibition effects of ciprofloxacin antibiotics at 24 and 20 mm, respectively.

 

Keywords :

Nanoparticles; biosynthesis of MgO NPs; Aspergillus niger; antimicrobial activity.

 

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