European Journal of Medicinal Plants, ISSN: 2231-0894,Vol.: 17, Issue.: 4
Antioxidant Activity of Coula edulis Baill. Seed Extracts
Basil Nse Ita1* and Gloria Ihuoma Ndukwe2 1Department of Chemistry, University of Uyo, Uyo, Nigeria. 2Department of Chemistry, Rivers State University of Science and Technology, Nkpolu-Oroworukwo, Port Harcourt, Nigeria.
Basil Nse Ita1* and Gloria Ihuoma Ndukwe2
1Department of Chemistry, University of Uyo, Uyo, Nigeria.
2Department of Chemistry, Rivers State University of Science and Technology, Nkpolu-Oroworukwo, Port Harcourt, Nigeria.
(1) Marcello Iriti, Professor of Plant Biology and Pathology, Department of Agricultural and Environmental Sciences, Milan State University, Italy.
(1) Alejandro Hurtado Salazar, Universidad de Caldas, Colombia, USA.
(2) Nathaniel Owusu Boadi, Kwame Nkrumah University of Science and Technology, Ghana.
Complete Peer review History: http://www.sciencedomain.org/review-history/17729
Aim: In this work, the antioxidant activity of organic and aqueous extracts of seed of Coula edulis Baill. was evaluated.
Study Design: Seeds of Coula edulis were collected, authenticated and extracted with acetone, ethanol and water respectively. The resulting extracts were used for antioxidant assay.
Place and Duration of Study: This work was done at the Department of Chemistry, University of Uyo between November 2015 and July 2016.
Methodology: Air-dried seeds of Coula edulis (800 g) were pulverized and macerated with 2.5 L of acetone, ethanol and water separately for 24 hrs at room temperature and then filtered. The acetone and ethanol filtrates were concentrated in vacuo, while the aqueous extract was freeze-dried to obtain the crude acetone (AcE), ethanol (EtE) and aqueous (AqE) extracts respectively. Content of total phenolics, flavonoids and vitamin C in the extracts were determined. Also, the DPPH and ABTS scavenging activity as well as the metal chelating and reducing power of the extracts were also evaluated.
Results: Our results indicated that Coula edulis seed is a rich source of total phenolics, flavonoids and vitamin C. Amongst the tested extracts, the acetone extract contained the highest amount of total phenolics (28.3 mgGAE/g), could chelate Fe2+ ions more effectively (IC50 = 46.2 μg/mL) and showed the best DPPH radical scavenging ability (IC50 = 19.2 μg/mL). The aqueous extract was the richest in flavonoids and vitamin C. Also, it showed the best reducing power and ABTS radical scavenging abilities with an IC50 of 25.2 μg/mL.
Conclusion: Coula edulis seed is a good source of natural antioxidants that can be exploited in food and therapeutic applications.
Coula edulis; antioxidant activity; metal chelating activity; ferric reducing activity.
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DOI : 10.9734/EJMP/2016/30762Review History Comments