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European Journal of Medicinal Plants, ISSN: 2231-0894,Vol.: 17, Issue.: 3

Original-research-article

Haematopoietic Enhancing Effect of Ethanolic Seed Extract of Citrullus lanatus (Watermelon) on Bone Marrow of Wistar Rats

 

E. Finbarrs-Bello1*, N. J. Nto2, I. T. Ikele2, M. I. Sani3 and V. Atuadu1

1Department of Anatomy, Faculty of Basic Medical Science, Enugu State University of Science and Technology (ESUT), Enugu, Nigeria.

2Department of Anatomy, University of Nigeria, Enugu Campus (UNEC), Enugu State, Nigeria.

3Department of Chemistry, School of Sciences, Federal College of Education, Zaria, Kaduna State, Nigeria.

Article Information
Editor(s):
(1) Marcello Iriti, Professor of Plant Biology and Pathology, Department of Agricultural and Environmental Sciences Milan State University, Italy.
Reviewers:
(1) T. T. Sreelekha, Regional Cancer Centre, Trivandrum, Kerala, India.
(2) Esmeralda Carrillo Delgado, University of Granada, Spain.
Complete Peer review History: http://www.sciencedomain.org/review-history/17526

Abstracts

Aim: To evaluate the haematopoietic property of citrullus lanatus seed extract by determining the phytochemical, cytological and histological profiles on the bone marrow of wistar rats. 

Methodology: Phytochemical properties were determined using a UNICAMM 969 atomic absorption spectrophotometer and functional groups were analyzed using Furrier Transform Infrared ray (FTIR). Fifteen (15) wistar rats average weights of 150 g were divided into three groups A, B, and C of 5 rats each. Group A (controls) received 0.1 ml saline while groups B and C received 100 mg/kg and 200 mg/kg of ethanolic seed extract of Citrullus lanatus respectively for 14 days. Thereafter, the animals were sacrificed under anaesthesia using ketamine 50 mg/kg. Bone marrow was collected from the femoral shaft and stained using haematoxylin and eosin (H & E) and its smear was also stained cytologically using May-Grünwald-giemsa stain.

Results: Atomic Absorbance Spectrum (AAS) revealed concentrations of 754.20 µg/kg, 53.70 µg/kg, 45.10 µg/kg, 24.20 mg/kg 11.80 µg/kg and 1.10 µg/kg for Iron, Zinc, Lead, Copper, Nickel and Cadmium ions respectively. The FTIR revealed presence of methyl, aldehyde, acetyl, carboxyl and ether groups. Bone marrow histology from groups B and C show greater cellularity compared to the control (group A). The cytology revealed more of myeloid precursors in the control and erythroid lymphoid precursors in the extract groups.

Conclusion: Citrullus lanatus seed extract has physiochemical properties which support its hematopoietic effect on the bone marrow and can be use as blood supplement.

Keywords :

Citrullus lanatus; bone marrow; histology; cytology; phytochemistry; hematopoietic.

Full Article - PDF    Page 1-7

DOI : 10.9734/EJMP/2016/30452

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