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International Journal of Biochemistry Research & Review, ISSN: 2231-086X,Vol.: 15, Issue.: 1

Original-research-article

Heavy Metals Effect on the Activity and Kinetics of Peroxidase Enzyme in Crude Extracts of Rosmarinus officinalis and Eruca sativa

 

Omar M. Atrooz1*, Manar A. Al-Btoush1 and Ibrahim M. Al-Rawashdeh2

1Department of Biology, Mutah University, P.O.Box 7, Mutah, Jordan.

2Department of Biological Science, Al-Hussein Bin Talal University, P.O.Box 20, Maan, Jordan.

Article Information
Editor(s):
(1) Hector M. Mora Montes, Department of Biology, University of Guanajuato, Mexico.
Reviewers:
(1) Flávio Henrique Silveira Rabêlo, University of São Paulo, Brazil.
(2) Naeem Khan, Kohat University of Science and Technology, Pakistan.
Complete Peer review History: http://www.sciencedomain.org/review-history/17466

Abstracts

The catalytic activities and kinetic parameters of the enzyme peroxidase (POX), extracted from two plant species namely Rosmarinus officinalis and Eruca sativa, were investigated in the presence and absence of various heavy metals. The activation effects of heavy metals Co2+, Fe3+ and Pb2+ have performed a noncompetitive inhibition on the enzyme activity in the crude extract of Eruca sativa. In contrast, Cd2+, Ni+ and Cu2+ have an uncompetitive inhibition for the two selected plants. Furthermore, Fe3+, Al3+, Pb2+ and Mo2+ were found to be acted as noncompetitive inhibitors on the enzyme activity in the crude extract of Rosmarinus officinalis. Presence of heavy metals altered the enzyme activity by acting as uncompetitive or noncompetitive inhibitors depending on the type of heavy metals.

Keywords :

Relative activities; uncompetitive inhibition; noncompetitive inhibition.

Full Article - PDF    Page 1-8

DOI : 10.9734/IJBCRR/2016/30399

Review History    Comments

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