American Chemical Science Journal, ISSN: 2249-0205,Vol.: 3, Issue.: 4 (October-December)
A Combined Experimental Green Flow-Injection Procedure and Computational Analysis to Determine Amino Acids
Samy Emara1*, Tarek Aboul-Fadl2, Abdel Nasser El-Shorbagy3, Maha Kamal4, Walaa Zarad1 and Mohamed Abdel Kawi5 1Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Misr International University, Km 28 Ismailia Road, Cairo, Egypt.
2Department of Medicinal Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt.
3Department of Pharmaceutical Organic Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt.
4Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Modern Sciences and Arts University, 26 July Mehwar Road intersection with Wahat Road, 6 October City, Egypt.
5Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El Aini St., Cairo 11562, Egypt.
Samy Emara1*, Tarek Aboul-Fadl2, Abdel Nasser El-Shorbagy3, Maha Kamal4, Walaa Zarad1 and Mohamed Abdel Kawi5
1Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Misr International University, Km 28 Ismailia Road, Cairo, Egypt.
(1) Hortensia Rodríguez, Laboratory Dr. Albericio, Chemistry and Molecular Pharmacology Programme, Institute for Research in Biomedicine, Parc Científic de Barcelona, Baldiri Reixac 10, 08028 Barcelona, Spain.
Complete Peer review History: http://www.sciencedomain.org/review-history/1697
Aim: Methods of pollution reduction and human health protection would be more successful if the fields of green analytical chemistry and computational analysis could come together. This overlap would help to minimize, or even eliminate the use and generation of hazardous substances. The goal of this study was to identify a way of further developing the fields of green analytical chemistry and computational analysis.
Place and Duration of Study: Misr International University, between April 2011 and November 2012.
Methodology: A single-line flow-injection (FIA) packed-reactor technique has been developed for the analysis of amino acids. Amino acids were injected into a flowing stream of distilled–deionized water, carried through the packed reactor of CuO followed by UV detection. The flow rate was 1.25 mL/min and the column temperature was ambient (25 °C). New mathematical models for correlations of the physicochemical parameters of the CuII-amino acid complexes with analytical parameters such as lower limit of detection (LOD), lower limit of quantitation (LOQ) as well as lower limit of linearity range (LL) and higher limit of linearity range (HL) were employed.
Results: The developed procedure can accommodate sample frequency of 60 sample/hrand a recovery% ranging from 98.800% to 100.260%. A good accordance was observed between the computed values of LOD, LOQ, LL and HL and the observed results.
Conclusion: An extremely simple environmentally friendly analytical procedure including packed-reactor FIA technique has been developed and evaluated for the routine quality control of amino acids. The developed method has excellent sensitivity and accuracy, as well as, it required relatively simple and inexpensive instrumentation. A good correlation between the computed values and the FIA results suggests that the mathematical models are reliable, and hence provide a theoretical basis for the determination of amino acids.
Flow injection analysis; CuO packed reactor; spectrophotometric detection; amino acids; computational analysis.
Full Article - PDF Page 419-433
DOI : 10.9734/ACSJ/2013/4923Review History Comments