British Microbiology Research Journal, ISSN: 2231-0886,Vol.: 1, Issue.: 3 (July-October)
Original Research Article
The Structure and Metabolic Diversity of Population of Pea Microsymbionts Isolated from Root Nodules
Jerzy Wielbo1*, Monika Marek-Kozaczuk1, Andrzej Mazur1, Agnieszka Kubik-Komar2 and Anna Skorupska1
1Department of Genetics and Microbiology, Maria Curie-SkÅ‚odowska University, Akademicka 19 str., 20-033 Lublin, Poland;
2Applied Mathematics and Informatics, Lublin University of Life Sciences, Akademicka 13 str., 20-950 Lublin, Poland.
Aims: The study of strains belonging to local rhizobial population, concerning their diversity in the genetic, metabolic and symbiotic properties, and their prevalence in the microsymbiont population.
Methodology: 257 rhizobial isolates recovered from nodules of five pea (Pisum sativum cv. Ramrod) plants grown at one site were classified using PCR-RFLP analysis of 16-23S rRNA ITS. After that, for representative group of 55 strains, 16-23S rRNA ITS region was sequenced, nodA-F region was analyzed by PCR-RFLP and sequencing, metabolic capabilities were studied using Biolog`s and growth tests and symbiotic performance in plant tests were assayed.
Results: Individual plants were infected by numerous and diverse strains, however, in the entire sampled population of microsymbionts, only three large clusters of strains could be distinguished on the basis of PCR-RFLP and sequence analyses of 16S-23S rRNA ITS region. Rhizobium strains belonging to different groups varied in plasmid number and the amount of plasmid DNA, utilization of carbon and energy sources, growth on soil extract-based media and the ability for symbiotic plant growth promotion. The most numerous group of the isolates was characterized by the high plasmid DNA content, low number of utilized sugar substrates, and comprised numerous strains with low symbiotic efficiency.
Conclusion: Sampled population of pea microsymbionts had its own characteristic structure with clearly distinguishable sub-populations, composed of numerous strains - probably descendants of a few old lineages, which diversified in the lapse of time. These strains are still competing during root nodule colonization, resulting in the symbiosis of individual pea plants with broad spectrum of different Rhizobium strains.
Rhizobium; pea microsymbionts; population structure; diversity;
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DOI : 10.9734/BMRJ/2011/412