British Journal of Medicine and Medical Research, ISSN: 2231-0614,Vol.: 3, Issue.: 4 (October-December)
Tissue Biomarkers in the Early Detection of Hepatocellular Carcinoma among Egyptian Patients with Chronic Hepatitis C: A Possible Genetic Profile
Hassan El-Garem1, Hanan Abdel- Hafez1*, Ahmed Foaud1, Wafaa Al Akel1, Mohey EldienAtia2, Mona Salah3, Hany Khatteb4, Heba Osman1, Khaled Ragab2 and Naglaa Zayed1 1Endemic Medicine and Hepatology Department Faculty of Medicine, Cairo University, Egypt.
2Tropical Department, Theodor Bilharz Research Institute, Egypt.
3Clinical Pathology Department,Faculty of Medicine, Cairo University, Egypt.
4Pathology Department, Faculty of Medicine, Cairo University, Egypt.
Hassan El-Garem1, Hanan Abdel- Hafez1*, Ahmed Foaud1, Wafaa Al Akel1, Mohey EldienAtia2, Mona Salah3, Hany Khatteb4, Heba Osman1, Khaled Ragab2 and Naglaa Zayed1
1Endemic Medicine and Hepatology Department Faculty of Medicine, Cairo University, Egypt.
(1) Philippe E. Spiess, Department of Genitourinary Oncology, Moffitt Cancer Center, USA and Department of Urology and Department of Oncologic Sciences (Joint Appointment), College of Medicine, University of South Florida, Tampa, FL, USA.
(2) Salomone Di Saverio, Emergency Surgery Unit, Department of General and Transplant Surgery, S. Orsola Malpighi University Hospital, Bologna, Italy.
(3) Dmitry A. Kuznetsov, Department of Medicinal Nanobiotechnologies, N. I. Pirogoff Russian State Medical University, Ostrovityanov St. 1, Moscow 117997, Russia and N. N. Semenov Institute for Chemical Physics, Russian Academy of Sciences, Kosygin St. 4, Moscow 119991, Russia.
Complete Peer review History: http://www.sciencedomain.org/review-history/1513
Background and Study Aims: Gene expression of biomarkers involved in hepatocarcinogensis could be used for the early diagnosis of hepatocellular carcinoma (HCC).
Aim: To evaluate the hepatocyte expression of Glypican-3 (GPC-3), paternally expressed gene 10 (PEG-10), Midkine (MDK), Serpin peptidase inhibitor (SERPINI1), and Ubiquinol-cytochrome (QP-C) which can represent a possible genetic profile among hepatitis C virus (HCV)-related HCC patients.
Patients and Methods: This prospective study was conducted on 70 Egyptian patients with HCV-related chronic liver disease and HCC patients. Patients were categorized into chronic HCV group (n=25), post-HCV cirrhosis group (n=24), HCC group (n=21) in addition to 7 healthy individuals who were candidates for living-donor related transplantation. Liver tissue obtained from all patients was subjected to total RNA extraction, reverse transcription of extracted RNA into cDNA and finally tissue expression of GPC-3, MDK, PEG-10, SERPINI1 and QP-C by qRT-PCR was assessed in each group.
Results: A significant increase in hepatocyte expression of GPC-3, MDK, SERPINI1, and QP-C was detected in cancerous compared to non-cancerous liver tissue; in contrast, PEG-10 was significantly expressed in chronic HCV patients. The ROC curves was able to identify best cutoff values, sensitivity and specificity for GPC-3 (7.26, 81%, 58%), SERPINI1 (0.16, 80%, 70%), MDK (3.8, 60%, 70%) and QP-C (0.45, 65%, 79%) respectively. There was no significant correlation between the tissue expression of these biomarkers and the size of hepatic focal lesion or AFP levels.
Conclusion: Hepatocyte expression of GPC-3, MDK, SERPINI1, and QP-C could represent a potential genetic profile for the early detection of HCC.
GPC-3; MDK; SERPINI1; PEG; QP-C; HCC; HCV; Egypt.
DOI : 10.9734/BJMMR/2013/4187Review History Comments
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