Quick Menu

Upcoming Journals

Annual Review & Research in Biology

Annual Review & Research in Biology, ISSN: 2231-4776,Vol.: 3, Issue.: 4 (October-December)

Research Paper

Detection of Common Transgenic Elements from Soy Sauce Samples by PCRs

 

Ping Yu1* and Chunyan Yu1

1College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, Zhejiang Province, People’s Republic of China.

 

Abstracts

 

Aims: To establish PCR procedures for detecting common transgenic elements from soy sauce samples.
Methodology: Soy sauce samples, Haitian, Jiajia, Taitaile and Lijinji, and common transgenic elements from them, the CaMV35S promoter, the NOS terminator, and the Cp4-EPSPS, were chosen to establish PCR procedures. Genomic DNAs from soy sauce samples were extracted by the improved CTAB method. Primers for amplifying transgenic elements were designed and transgenic elements were amplified with extracted genomic DNAs as the templates. Amplified products were detected and analyzed using 1% agarose gel electrophoresis.
Results: The lectin gene from four samples, the NOS terminator from a sample and the Cp4 -EPSPS from two samples could be amplified by the established PCR procedure. The CaMV35S promoter sequence could be amplified from three soy sauce samples by the established nested PCR procedure.
Conclusion: This study lays a good foundation for developing the reagent kit for detecting common transgenic elements from soy sauce samples.

 

Keywords :

Transgenic elements; soy sauce; nested PCR; detection.

 

Full Article - PDF    Page 524-529    Article Metrics

Review History    Comments

Search this site

Advanced Search

Announcement & News

Nature (Impact Factor: 41.6) confirmed high standard of SDI journal and its editors

We are happy to inform that Nature (Impact Factor: 41.6) confirmed high standard of SDI journal and ...

ISI Thomson Reuters selected British Journal of Pharmaceutical Research for Emerging Sources Citation Index

We are delighted to inform that ISI Thomson Reuters selected British Journal of Pharmaceutical Resea...

SCOPUS selected Annual Research & Review in Biology (ARRB)

We are delighted to inform that famous indexing organization SCOPUS (from Elsevier) selected  A...

Index Copernicus Evaluation Result Released

We are delighted to inform that Index Copernicus (a leading indexing organization from Pol...

Journal Repository (JR): Permanent Digital Archiving of SDI journals

SDI is happy to announce that all our journals are now permanently archived in Journal Repository (J...

SDI journal got 35th ranking in Publons

We are delighted to announce (as of 04/01/2016) that British Journal of Medicine and Medical Re...

Growth of SDI and world publication market

As of 2014, total 25,064 journals are competing in World market of journal publication. In 2011, tot...

Science (IF: 31) report confirmed the high standard of SDI journal

As per a recent report (Link) of Science journal (present Impact factor 31), one of our journal (Bri...

SDI introduced Post-publication peer review by its comment section

SDI journals encourage Post-publication peer review by its comment section   Policy details a...

SDI promotes transparent Advanced OPEN peer review

We have migrated to transparent and toughest ‘Advanced OPEN peer review’ system (...



SCIENCEDOMAIN Awards

  • No Awards listed.

Browser Compatibility : Mozila firefox, Google Crome and IE 7 & above. Creative Commons License Terms & Condition   |   Privacy Policy   |   Join Us   |   Help   |   Contact Us
© Copyright 2010-2018, SCIENCEDOMAIN international. All rights reserved.