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British Journal of Medicine and Medical Research, ISSN: 2231-0614,Vol.: 13, Issue.: 5

Original-research-article

Comparative Studies of Therapeutic Effect of   Leaves, Stem Bark and Root Bark Extracts of Afzelia africana (Smith) in Mice Challenged with Trypanosoma brucei brucei

 

M. H. Garba1,2*, A. Y. Kabir2, Y. Garba3, M. A. Yusuf1, M. Inuwa1, B. J. Lekene1, O. A. Ibrahim4 and L. M. Hafsa1

1Department of Animal Production Technology, Federal College of Wildlife Management, P.M.B. 268, New Bussa, Niger State, Nigeria.

2Department of Biochemistry, Trypanosomiasis and Malaria Research Unit, Federal University of Technology, P.M.B. 65, Minna, Niger State, Nigeria.

3Department of Biological Sciences, Federal College of Education, P.M.B.39, Kontagora, Niger State, Nigeria.

4Department of Forestry Technology, Federal College of Wildlife Management, P.M.B. 268, New Bussa, Niger State, Nigeria.

Article Information

Editor(s):

(1) Alex Xiucheng Fan, Department of Biochemistry and Molecular Biology, University of Florida, USA.

(2) Chan-Min Liu, School of Life Science, Xuzhou Normal University, Xuzhou City, China.

(3) Chan Shen, Department of Biostatistics, MD Anderson Cancer Center, University of Texas, USA.

Reviewers:

(1) K. W. Nkpaa, University of Port Harcourt, Port Harcourt, Nigeria.

(2) S. Sivajothi, Sri Venkateswara Veterinary University, India.

(3) Hazem Mohammed Ebraheem Shaheen, Damanhour University, Egypt.

Complete Peer review History: http://www.sciencedomain.org/review-history/13018

Abstracts

Background: Traditional and complimentary health care is inarguably the system most close to homes, accessible and affordable. It is also culturally acceptable and trusted by large numbers of people. The affordability of most traditional medicines makes them all the more attractive at a time of soaring health-care costs, neglect of orphaned/non profitable diseases and nearly universal austerity.

Aim: Aqueous leaf, stem bark and root bark extracts were evaluated for their anti trypanosomal effect in experimental trypanosomiasis with a view to come up with a phytomedicine that is efficacious, available, accessible and non-toxic to both humans and animals.

Study Design: Complete randomized clinical trial design was used in the experiment.

Methodology: Ninety five (95) mice were grouped into three (I, II, III) of thirty mice each (with sub groups A, B, C, D, E, and F consisting of five mice each) to which the leaf, stem bark and root bark extracts were administered at a dose of 100, 200, 300, 400 mg/Kgbw, while the remaining five mice served as the control for all the groups.

Results: The aqueous leaves extract at doses of 100, 200 and 300 mg/Kg bw portrayed very low activities except for the 400 mg/Kg bw that displayed a sustained Trypanostatic effect. The aqueous stem bark extract, at doses of 100 and 200 mg/Kg bw portrayed trypanostatic effect while doses of 300 and 400 mg/Kg bw effectively cleared the parasites from circulation on the 13th and 17th days into the treatment respectively. Three and two of treated mice survived and remained apparasitaemic for up to 120 days and beyond in the group treated with 300 and 400 mg/kg bw respectively. In the group treated with the root bark extract, the mice on a dose of 100 mg/Kg bw died some few days into the experiment (6th day) while the dose of 200 mg/Kgbw sustained the animals until the 19th day. Doses of 300 and 400 mg/Kg bw were observed to clear the parasites in circulation after sustained administration for 23 and 16 days respectively.

Conclusion: This study has demonstrated the potency of the stem bark and root bark crude extracts of Afzelia africana in treating experimental trypanosomiasis and can thus be further purified and packaged as phytomedicine against this dreaded but neglected disease.

Keywords :

Trypanosomiasis; trypanosomes; Afzelia africana; phytomedicine; chemotherapy.

Full Article - PDF    Page 1-14

DOI : 10.9734/BJMMR/2016/23033

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