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British Journal of Medicine and Medical Research, ISSN: 2231-0614,Vol.: 1, Issue.: 3 (July-September)

Short Communication

Paraffin Sections of Sputum Block: Description of a New Method for Pathological and Molecular Study

Arshad Rahmani1,2&3*, Mohammad Alzohairy1, Habeeb Khadri1, Moshahid A. Rizvi2 and Ashish Mandal3

1Department of Medical Laboratory Science, College of Applied Medical Sciences, Qassim University, Kingdom of Saudi Arabia.
2Department of Biosciences, Jamia Millia Islamia, New Delhi, India.

3Department of Pathology, Maulana Azad Medical College, New Delhi, India.


Aim: This study was designed to enhance the scope of sputum analysis by using it as a clinical tool in gene/protein expression, by making the paraffin embedded blocks.
Methodology: The specimens were prepared as smear and cell blocks for cytopathologic examination. The preparation of paraffin-embedded block from sputum samples employs fixation and dehydration of the cell specimens. The sputum specimen is first exposed to a suitable fixating agent, Formalin, and graded acetone was employed to dehydrate the samples and saved as pellet. Paraffin blocks containing embedded pellet were taken out gently from the tube. The solidified paraffin-embedded tissue blocks through this novel approach were found to be easy to process for immunohistochemistry and molecular analysis.
Results: Immunohistochemistry staining was performed on paraffin section of sputum block for Cytokeratin (CK) and was found to be easy to process for immunohistochemistry.
Conclusion: The sputum block preparation is feasible and non-invasive, can be useful to identify new biomarkers of exposure or susceptibility in patients with lung pathology to enhance the understanding of airways changes due to different etiological factors and may be useful to find new biomarkers in order to assess and monitor early lung damage.

Keywords :

Sputum block; immunohistochemistry; molecular; cytokeratin;

Full Article - PDF    Page 141-145

DOI : 10.9734/BJMMR/2011/421

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