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British Journal of Medicine and Medical Research, ISSN: 2231-0614,Vol.: 3, Issue.: 4 (October-December)

Original-research-article

Distribution of Telomere Length in the Cord Blood of Chinese Newborns

 

Shi-Ni Lim1, Zaheedah Yahya2, Dimphy Zeegers2, Thiha Moe3, Ei Ei Phyo Kyaw3, George SH Yeo3, M Prakash Hande2 and Ene-Choo Tan1,4*

1KK Research Centre, KK Women’s and Children’s Hospital, Singapore.
2Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore.
3Maternal-Fetal Medicine, KK Women’s and Children’s Hospital, Singapore.
4Office of Clinical Sciences, Duke-NUS Graduate Medical School, Singapore.

Article Information

Editor(s):

(1) Salomone Di Saverio, Emergency Surgery Unit, Department of General and Transplant Surgery, S. Orsola Malpighi University Hospital, Bologna, Italy.

Reviewers:

(1) Dean H. Betts, University of Western Ontario, Canada.

(2) Anonymous.

(3) Prathibha Shetty, India.

(4) David T. Harris, University of Arizona, USA.

Complete Peer review History:http://www.sciencedomain.org/review-history/1101

Abstracts

Aims: We studied the variability in telomere length in cord blood collected from newborns of different birth weights and gestational ages.
Study Design: Prospective cohort study.
Place and Duration of Study: Samples were collected from KK Women’s and Children’s Hospital between March 2011 and March 2012 and the terminal restriction fragment assays (TRF) were performed at the Department of Physiology, National University of Singapore.
Methodology: Cord blood samples were prospectively collected in EDTA or heparin tubes for deliveries from Chinese parents. TRF assays were performed on genomic DNA extracted from whole blood. Data was collected for birth weight, gestational age, and maternal age. Variance analyses of telomere lengths and correlation coefficients were calculated using Statistical Package for the Social Sciences (SPSS).
Results: The birth weight of the samples collected ranged from 0.61 kg to 5.18 kg with gestation age from 196 to 288 days. TRF results from 184 samples (96 males, 88 females) showed that there was a wide range from 6.6 kbp to 19.2 kbp. The mean TRF length was 12.64 kb (males: 12.33 kb ± 2.50; females: 12.99 kb ± 2.35). There was no statistically significant correlation of TRF with birth weight, gestation age or maternal age. There was highly significant correlation of birth weight with gestational age (P=0.00).
Conclusion: Our results showed no correlation of either gestational age or birth weight with telomere length as measured by TRF assay.

Keywords :

Birth weight; Chinese; gestational age; maternal age; telomere length.

Full Article - PDF    Page 1004-1014

DOI : 10.9734/BJMMR/2013/2676

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