European Journal of Food Research & Review, ISSN: 2231-0878,Vol.: 1, Issue.: 1 (April-June)
Simultaneous Determination of Erythromycin A in Giant Prawn and Tilapia in Mekong Region by Stripping Square Wave Voltammetry
Nguyen Phuoc Minh1*, Tran Bich Lam1, Nguyen Trong Giao1 1Department of Food Technology, Ho Chi Minh City University of Technology, 268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City, Vietnam
Nguyen Phuoc Minh1*, Tran Bich Lam1, Nguyen Trong Giao1
1Department of Food Technology, Ho Chi Minh City University of Technology, 268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City, Vietnam
Erythromycin A (EA) is now one of antibiotics limited in seafood products in general and in giant freshwater prawns (Macrobrachium rosenbergii) and tilapia (Oreochromis niloticus) in particular while exporting to the US, EU, Japan, Canada. There are many methods used for analyzing this antibiotic in these aqua species such as ELISA, HPLC, LC-MS/MS, GC-MS, etc. A new sensitive analytical approach for determination of erythromycin A using stripping square wave voltammetry at the slowly dropping mercury electrode was primarily developed and validated to quantify this antibiotic with simple and short time analysis, not too pure analytical solutions, not high price equipments. Electrochemical signals were measured at potential wave -1430 mV. The optimal experimental parameters for the method were: supporting electrolyte ammonium acetate 0.1 M, pH 8.0, the solvents for dissolving erythromycin standard: acetonitril, Vstart = -400 mV, Vstop = -1700 mV, Vstep = 6 mV, Vpulse = 40 mV, Tdrop = 5000 ms , Velctrolise = -1100 mV, Telectrolyse = 5 s. The method showed high recovery (85.07 ÷ 96.50 %), high sensitivity (lower limit of detection, LoD = 0.57 µg kg-1 in giant prawn and LoD = 0.52 µg kg-1 in tilapia) and high precision (RSD 0.91 ÷ 2.1 %) as well as excellent linearity (r2adjusted ≥ 0.99999).
Erythromycin A; giant freshwater prawn; tilapia; stripping square wave voltammetry; dropping mercury electrode
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